具體引用情況如下：

        “在標準條件下擴增的oAEC通過上調EMT轉錄因子自發(fā)獲得間質特性， 通過抑制EMT誘導機制比如TGF- β的自分泌（定量測定采用基諾萊斯科技有限公司生產(chǎn)的Nori Sheep TGF-β3 ELISA試劑盒）和細胞內相關信號的活化， P4可以阻止這種表型的變化。從細胞培養(yǎng)基中去掉P4然后細胞傳代一次，P4的這種作用仍然存在，并且P4可以很快逆轉擴增中正在進行EMT轉化的oAEC間質表型。此外，P4促進多能行基因的急性上調進而提高基底和LPS誘導的oAEC抗炎反應，表現(xiàn)為抗炎細胞因子表達增加（定量測定采用基諾萊斯科技有限公司生產(chǎn)的Nori Sheep IL-4 and IL-10 ELISA試劑盒）和促炎性細胞因子表達降低。綜合上述結果，P4對于維持oAEC的上皮表型提高其生物學特性至關重要，因此，為了提高這種上皮干細胞的治療潛力，我們提出了一種新的細胞培養(yǎng)方法。”

        《自然》雜志在國際上影響力巨大，其影響因子高達38.138，基諾萊斯產(chǎn)品被其引用有重大的現(xiàn)實意義，標志著公司免疫分析技術已達到世界先進水平，產(chǎn)品質量也得到國際上的充分肯定，極大地提升了公司的知名度，有助于公司產(chǎn)品得到更好的推廣和發(fā)展。目前,基諾萊斯正在推行心梗早期預警診斷項目，預計相關產(chǎn)品將在兩年內問世。基諾萊斯將不斷向成為心血管病早期預警診斷一體化POCT智能系統(tǒng)供應商的目標奮進，為推動我國大健康產(chǎn)業(yè)發(fā)展做出積極貢獻。

附件：原英文文獻

Title: Progesterone prevents epithelialmesenchymal transition of ovine amniotic epithelial cells and enhances their immunomodulatory properties

Published at: Nature, 19-Jun-17, doi:10.1038/s41598-017-03908-1.

Authors: Angelo Canciello 1, Valentina Russo1, Paolo Berardinelli1, Nicola Bernabò1, Aurelio Muttini1, Mauro Mattioli2 & Barbara Barboni1

1Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Via Renato Balzarini 1, 64100, Teramo, Italy. 2Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise (IZSAM) “G. Caporale”, Campo Boario, 64100, Teramo, Italy. Correspondence and requests for materials should be addressed to A.C. (acanciello@unite.it).

 The in vitro expansion is detrimental to therapeutic applications of amniotic epithelial cells (AEC), an emerging source of fetal stem cells. This study provides molecular evidences of progesterone (P4) role in preventing epithelial-mesenchymal transition (EMT) in ovine AEC (oAEC). oAEC amplified under standard conditions spontaneously acquired mesenchymal properties through the up-regulation of EMT-transcription factors. P4 supplementation prevented phenotype shift by inhibiting the EMT inducing mechanism such as the autocrine production of TGF-β (quantified by Nori Sheep TGF-β3 ELISA Kit, Genorise Scientific, Inc.) and the activation of intracellular related signaling. The effect of P4 still persisted for one passage after steroid removal from culture as well as steroid supplementation promptly reversed mesenchymal phenotype in oAEC which have experienced EMT during amplification. Furthermore, P4 promoted an acute up-regulation of pluripotent genes whereas enhanced basal and LPS-induced oAEC anti-inflammatory response with an increase in anti-inflammatory (quantified by Nori Sheep IL-4 and IL-10 ELISA Kits, Genorise Scientific, Inc.) and a decrease in pro-inflammatory cytokines expression. Altogether, these results indicate that P4 supplementation is crucial to preserve epithelial phenotype and to enhance biological properties in expanded oAEC. Therefore, an innovative cultural approach is proposed in order to improve therapeutic potential of this promising source of epithelial stem cells.